Figure 1. A) 272 bp insert DNA sequence from clone pGEM-OnCA09 showing three tandem GAAAAT repeats (underlined) and primer binding sites (underscored by arrows indicating direction) used to PCR amplify the locus Ostrinia nubilalis W-chromosome marker number 1 (ONW1; GenBank accession: AF442958). B) Multiple DNA sequence alignment of the PCR amplified O. nubilalis Z-chromosome linked marker number 1 (ONZ1) alleles. Consensus sequence of imperfect alleles, CAYCARCGTCACTAA (underlined nucleotides optional), and primer binding sites (arrows indicating direction) are underscored, and - representing an invariable nucleotide identical to clone OnCA01, and * is a deletion.