Figure 5. Northern blot analysis of AmTRF expression in heads and abdomens of juvenile hormone-treated honeybees. Newly emerged individuals were treated with JH as described in materials and methods and snap frozen 24 hrs later. For comparison, the expression patterns of SPARC, a member of the cell adhesion molecules family (Kucharski and Maleszka, 2002) is also shown. The relative intensity of hybridisation signals was calculated using ImageQuant software as described in materials and methods.