Figure 1. SDS-PAGE of centrifugal supernatants (s) and pellets (p) of soft tissue/hemolymph homogenates of Manduca sexta were run on 4-15%, precast minigels. Stages extracted include newly ecdysed pupae (P1) and adults one, four and six days after eclosion (A1, A4, A6). Serving as molecular weight standards were the major proteins in a hemolymph/soft tissue extract of diapausing Hyalophora cecropia pupae (left lane in A). The H. cecropia preparation includes 200-kDa apolipophorin I (lp1), 180-kDa large subunit of Vg (vg1), unresolved 73-79-kDa subunits of ArH, V-MtH and M-MtH (hex), and 18-kDa apolipophorin III (lp3). The heavy band lying above hex includes 85-kDa subunits of the riboflavin-binding hexamerin, a major H. cecropia hexamerin that is not detected in M. sexta.