Figure 3. Southern blot analysis of the rice weevil polygalacturonase gene. Genomic DNA was extracted from either whole adult insects (left panels) or legs carefully dissected from insects (right panels) and was then digested with the indicated restriction endonucleases. The cDNA shown in Figure 1 was radiolabeled and used as the probe. E, Eco RI; H, Hind III; X, Xba I