Figure 2. Multiple alignment of the conserved domain of polygalacturonases from various kingdoms. (*) denotes identical residues, (:)denotes highly conserved residues, and (·) indicates active site residues identified by Pickersgill (1998). Dashes represent gaps that were inserted to preserve the alignments. S.oryzae, Sitophilus oryzae (accession number AF207068); A.niger, Aspergillus niger (P26213); S.cerev., Saccharomyces cerevisiae (P47180); maize, Zea mays (CAA40910); tomato, Lycopersicon esculentum (M37304); E.carot., Erwinia carotovora (3891426); P.solan., Pseudomonas solanacearum (M33692)