Table 2. Biological Process

Gene Ontology Terma Number of ESTs % of total ESTs representedb Number of contigs Number of singlets [p] Behavior 7 0.81% 1 4 [p] Cell Communication         [c] Cell adhesion 2 0.23% 0 2 [c] Cell-cell signaling 3 0.35% 0 3 [c] Response to external stimulus 19 2.20% 5 7 [c] Signal transduction 8 0.93% 0 8 [p] Cell Growth and/or maintenance         [c] General cell growh and/or maintenance 1 0.12% 0 1 [c] Cell cycle 12 1.39% 0 12 [c] Cell motility 10 1.16% 2 0 [c] Cell organization and biogenesis 60 6.94% 6 13 [c] Homeostasis 16 1.85% 4 2 [c] Membrane fusion 1 0.12% 0 1 [c] Metabolism         [i] Alcohol metabolism 18 2.08% 7 0 [i] Amine metabolism 2 0.23% 1 0 [i] Aromatic compound metabolism 3 0.35% 1 1 [i] Biosynthesis 18 2.08% 4 7 [i] Carbohydrate metabolism 4 0.46% 0 4 [i] Catabolism 4 0.46% 1 0 [i] Energy pathways 2 0.23% 1 0 [i] Lipid metabolism 1 0.12% 0 1 [i] Nucleobase, nucleoside, nucleotide and nucleic acid metabolism 36 4.17% 5 26 [i] Phosporous metabolism 126 14.58% 4 11 [i] Protein metabolism 393 45.49% 76 31 [i] Sulfur metabolism 2 0.23% 0 2 [c] Response to stress 1 0.12% 0 1 [c] Transport 56 6.48% 13 11 [p] Development         [c] Cell differentiation 8 0.93% 2 0 [c] Cell fate commitment 1 0.12% 0 1 [c] Embryonic development 2 0.23% 0 2 [c] Larval development 2 0.23% 1 0 [c] Morphogenesis         [i] Morphogenesis of an epithelium 1 0.12% 0 1 [i] Organogenesis         [ii] Histogenesis         [iii] Ectoderm development 2 0.23% 1 0 [ii] Imaginal disc development 2 0.23% 0 2 [ii] Muscle development 7 0.81% 3 0 [ii] Neurogenesis 23 2.66% 4 4 [ii] Trachael system development 1 0.12% 0 1 [c] Reproduction 9 1.04% 1 5 [p] Physiological processes         [c] Cuticle biosynthesis 1 0.12% 0 1 Totals 864   143 165

aClassification is hierarchial: idented terms are children [c] of parent terms [p] listed above. All functional assignments of Toxoptera citricida ESTs described here are the "inferred from electronic evidence" (IEA) using the top 5 BLASTX hits with an E-value of -10 generated from NCBI's nr database. The definition term associated with each sequence was entered into both FlyBase and AmiGO where the it was given a molecular function according to The Gene Ontology Consortium. b% of total ESTs represented was calculated using only those ESTs with a BLASTX hit with an E-value of -10 and of known protein function.