Table 1. Molecular Function

Gene Ontology Terma Number of ESTs % of total ESTs representedb Number of contigs Number of singlets [p] Antioxidant 1 0.06% 0 1 [p] Apoptosis 1 0.06% 0 1 [p] Binding         [c] General binding 8 0.50% 2 3 [c] Calcium ion binding 16 1.00% 3 5 [c] Carbohydrate binding 2 0.12% 1 0 [c] Drug binding 10 0.62% 1 1 [c] Heavy-metal binding 10 0.62% 3 0 [c] Hormone binding 1 0.06% 0 1 [c] Isoprenoid binding 7 0.44% 1 1 [c] Lipid binding 12 0.75% 3 2 [c] Neurotransmitter binding 2 0.12% 0 2 [c] Nucleic acid binding         [i] DNA binding 29 1.81% 4 9 [ii] Transcription factor 8 0.50% 1 6 [i] RNA binding 48 2.99% 9 18 [i] Nuclease activity 1 0.06% 0 1 [i] Transcription factor 6 0.37% 1 4 [i] Translation factor 27 1.68% 1 5 [c] Nucleotide binding 22 1.37% 3 10 [c] Odorant binding 1 0.06% 0 1 [c] Oxygen binding 14 0.87% 3 1 [c] Protein binding 60 3.74% 12 20 [c] Steroid binding 1 0.06% 0 1 [p] Cell Adhesion Molecule 1 0.06% 0 1 [p] Chaperone 26 1.62% 6 12 [p] Enzyme         [c] Helicase 2 0.12% 0 2 [c] Histone deactylase 1 0.06% 0 1 [c] Hydrolase         [i] General hydrolase 12 0.75% 3 6 [i] Acting on acid anhydrides 98 6.11% 21 21 [i] Acting on ester bonds 22 1.37% 4 14 [i] Acting on ether bonds 2 0.12% 0 2 [i] Acting on glycosyl bonds 1 0.06% 0 1 [i] Peptidase 51 3.18% 11 24 [c] Isomerase 15 0.94% 5 4 [c] Kinase 23 1.43% 5 11 [c] Ligase 19 1.19% 5 5 [c] Lyase 27 1.68% 6 9 [c] Molybdopterin cofactor sulfarase 1 0.06% 0 1 [c] Oxidoreductase 283 17.65% 31 51 [c] Small protein activating enzyme 1 0.06% 0 1 [c] Small protein conjugating enzyme 5 0.31% 1 3 [c] Transferase 66 4.12% 10 36 [p] Enzyme regulator 21 1.31% 3 11 [p] Protein degradation tagging 18 1.12% 4 8 [p] Signal transducer 13 0.81% 2 7 [p] Structural molecule         [c] Cuticular protein 84 5.24% 14 6 [c] Cytoskeleton protein 42 2.62% 3 2 [c] Muscle fiber 2 0.12% 1 0 [c] Ribosomal protein 337 21.02% 65 29 [p] Transcription regulation 16 1.00% 3 10 [p] Translation regulation 13 0.81% 5 1 [p] Transporter 114 7.11% 10 23 Totals 1603   266 395

aClassification is hierarchial: idented terms are children [c] of parent terms [p] listed above. All functional assignments of Toxoptera citricida ESTs described here are the "inferred from electronic evidence" (IEA) using the top 5 BLASTX hits with an E-value of -10 generated from NCBI's nr database. The definition term associated with each sequence was entered into both FlyBase and AmiGO where the it was given a molecular function according to The Gene Ontology Consortium. b% of total ESTs represented was calculated using only those ESTs with a BLASTX hit with an E-value of -10 and of known protein function.