Table 5. Electroporation of 3 and 4 day old embryos.

Experiments Age of embryos Assays Number of electroporated embryos Number of positive embryos Number of spots Mean number of spots Percentage of positive embryos Electrodes 1 4d 1 2 19 18 11 3 133 11 7 1.37 57.8 37.5 BTX 2 3d 1 2 2 3 4 10 10 15 10 2 0 0 2 2 0 0 4 0.2 0 0 0.4 20 0 0 20 Aluminium

Electroporation program : P'2. ADN: pBRJZ (0.5 µg/µl). Electrodes: aluminium, customised aluminium electrode set having 8 mm gap width BTX, microslide electrode set model 453 having 3.2 mm gap width.