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Table 2. Optimization of electroporation parameters.

Electroporation program Assay number Number of electroported embryos Number of positive embryos Percentage of positive embryos Number of positive spots Mean number of positive spots per embryo
P1
(5,10,25)*		 1
2		 6
6		 6
6		 100
100		 130
114		 21.6
19
P2
(5,50,250)		 1
2		 6
6		 6
6		 100
100		 470
296		 78.3
49.3
P3
(5,100,250)
 1
2
3		 6
6
6
 4
5
4		 66.6
83.3
66.6		 97
171
72		 16.16
28.5
12
P4
(10,50,250)		 1
2		 6
6		 6
5		 100
83.3		 34
65		 5.6
13
P5
(5,100,125)		 1
2		 6
8		 3
0		 50
0		 7
0		 1.16
0
P6
(5,50,125)		 1
 5
 0
 0
 0
 0
P7
(5,10,500)		 1
 6
 6
 100
 469
 78.1
P8
(5,50,500)

 1
2
3
4		 6
4
6
6		 1
3
6
5		 16.6
75
100
83		 4
87
164
53		 0.66
27.55
27.3
8.33
* : first number : number of pulses, second number : duration of one pulse, third number : voltage of the pulses. The frequency of the pulses was fixed at 1 hertz. For each assay the electrode set used was A13122001 except for the assays marked with the star which was done using the BTX microslide electrode set model number 453.
DNA : pBRJZ (0.5 µg/µl).
Number in bold and underlined are the markedely highest values.
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