Figure 1. Electrodes and culture of embryos. A : Custom electrode set made of 2 aluminium (U-section) bars glued to a microscope glass slide with epoxy-glue. Arrows show the epoxy walls making the slot leak proof. Circle surrounds the well of the agarose block containing embryos. The electrodes are 8 mm apart. B : Embryos inside the agarose wells. C : The block containing embryos is cut and directly put in a 35 mm Petri dish. D : 6 day old embryos in place on glass filter just after injection of the DNA solution containing 0.5% eosin (pinkish color of the embryos). E : Electrode set used to electroporate 6 day old embryos. Embryos are visible between the electrodes. Black arrows show the two platinum wires (diameter 0.3 mm). Arrow-heads show the soldered joint of electrodes on copper wires. Epoxy-glue (stars) was used to affix all parts on a microscope slide to get a leak proof slot.