Figure 4. (A) Cross-sections of female wings cultured with 20E. Black arrowheads indicate the position of the BL. Female P-0: P-0 female wing; floating hemocytes are visible (right photo, red arrowhead). Female 24 h: wing cultured for 24 h with 20E; plasmatocytes (right photo, red arrowhead) and granulocytes (right photo, yellow arrowhead) are visible in the area proximal to the BL. Female 38.5 h: wing cultured for 38.5 h with 20E. Phagocytosis (right photo, red arrowhead) are seen in areas both distal and proximal to the BL. Female 48 h: hemocytes are visible floating in the wing (right photo, red arrowhead). (B) Male 38.5 h: male wing cultured for 38.5 h with 20E; phagocytosis was observed only in the area distal to the BL (right photo, red arrowhead). Bars: 100µm.