Figure 3. TUNEL detection of apoptosis in O. recens wings. (a) Female wing at 3 h after pupation. Blue signals (indicating apoptotic cells) were seen in the area distal to the BL (white arrowhead). (b) Male wing at 6 h after pupation. Blue signals were seen in the area distal to the BL. (c) Female wing cultured for 6 h with 20E. Apoptotic features (light green indicated by small white arrowheads) were visible in areas both distal and proximal to the BL. (d) Male wing cultured for 6 h with 20E. Light green signals were visible only in the area distal to the BL. (e) Female wing cultured for 6 h without 20E. No signals observed. (f) Male wing cultured for 6 h without 20E. No signals. Bars: 1 mm.