Figure 2. Morphological changes in cultured pupal wings induced by ecdysteroid (20-hydroxyecdysone, 20E). All wings were laid within their cuticular pouch. (A) (a) Female wing cultured with 1 µg/ml 20E degenerated to one-fifth the size (arrowheads). (b) Male wing cultured with 1 µg/ml 20E showed only peripheral degeneration. (c) Female wing cultured without hormone. (d) Male wing cultured without hormone. (e) Female wings cultured with 1 µg/ml 20E for two days and stained with DAPI. (f) Male wing cultured with 1 µg/ml 20E for two days and stained with DAPI. (B) Female wings cultured with 0.1 µg/ml 20E for 2 days and stained with Nuclear Fast Red (g). (h) Magnified view of (g). Note that the peripheral structure distal to the BL (arrowheads) remains in this condition.