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Figure 1a
Figure 1b
Figure 1. . (A) (a and b): Adult O. recens. (a) Male whole view. (b) Female whole view. (c and d): Optical micrographs of the forewings of male (b) and female (c). (e and f): Dissected wings from pupae immediately after pupation (P-0) of male (e) and female (f). The bordering lacuna (BL) runs parallel to the periphery of pupal wings of both sexes. (B) Optical micrographs of the female pupal forewings at various stages, stained with methyl green. All wings were left in their cuticular pouch. (a and b): The pupal wings just after pupation (P-0). (b) Magnified view of the boxed area in (a). The BL (arrowhead) runs parallel to the wing edge. (c and d): Pupal wings at 1 day after pupation (P-1). No morphological change has occurred at this stage. (d) Magnified view of the boxed area in (c). (e and f): The pupal wings 36 h after pupation (P-1.5). Wing degeneration has occurred at this stage. (f) Magnified view of the boxed area in (e). The region distal to the BL (arrowhead) remains at this stage. (g and h): The wings at 2 days after pupation (P-2). The wing degeneration has completed at this stage. (h) Magnified view of the boxed area in (g). Scales have formed and long scales (arrowhead) are visible. In this figure and Figs. 2, 3 and 4, distal is to the right and anterior is to the top. Bars: 1 mm. After emergence, female wings do not inflate. We observed that the size of adult wings is same as the degenerated wings at the late pupal stages, and therefore reduction of the female wings occurs in the early pupal stages.
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