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Table 1 . "Artificial" flies were prepared by mixing RNA and DNA standards to target insect nucleic acid concentrations (n=3 unless noted in table) and processing as a sample. RNA and DNA concentrations (µg ml-1)plus or minus standard errors are reported. RNA/DNA ratios are calculated from initial estimated RNA/DNA. Percent recovery is percent of nucleic acid measured compared to actual. Mean values for the 5 experiments are given with standard errors.
Experiment RNA:DNA RNA
(µg ml-1)
% Recovery DNA
(µg ml-1)
% Recovery
1 4.17 0.97plus or minus0.002 96.5 0.25plus or minus0.003 106.3
2 1.25 0.186plus or minus0.006 93.0 0.193plus or minus0.004 121.0
3 6.25 0.901plus or minus0.012 90.1 0.20 (n=1) 126.2
4 1.25 0.201plus or minus0.015 100.5 0.166plus or minus0.001 103.7
5 6.25 1.07plus or minus0.013 107 0.174plus or minus0.002 108.7
Mean 97.4plus or minus2.9 113.8plus or minus4.4
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