Table 1 Significant differences in fluorescence intensity between substrates and between copper concentration in different larval body compartments of Chironomus luridus (2 way ANOVA) (see data in Figure 2).

Body compartments   Degrees of freedom F - ratio P Significance level Midgut Between substrates 1 23.9 0.0001 *** Between concentrations 2 0.97 0.39 n.s. Interaction 2 2.9 0.074 n.s. Hindgut Between substrates 1 0.6 0.0001 *** Between concentrations 2 2.17 0.136 n.s. Interaction 2 2.4 0.109 n.s. Tracheal gills (gills) Between substrates 1 13.6 0.0012 ** Between concentrations 2 3.65 0.041 * Interaction 2 5.9 0.0082 ** Fat body & muscles Between substrates 1 17.7 0.0003 *** Between concentrations 2 4.3 0.025 * Interaction 2 7.5 0.003 ** Malphighian tubules Between substrates 1 12.3 0.0018 ** Between concentrations 2 9.1 0.0011 ** Interaction 2 6.25 0.007 **