Figure 4. Expression of ferritin HCH messages and subunits in female Aedes aegypti fed various meals. A. Northern blot of total RNA prepared from mosquitoes 24 h after feeding, hybridized with a probe from the Fer HCH coding region and 3'UTR. B. Ethidium bromide stained rRNA on the Northern blot to show loading. C. Western blot of extracts (one insect per lane) prepared from mosquitoes 24 h after feeding and probed with antibodies against ferritin. Mosquitoes were sugar-fed or fed on the meals as indicated. Hb, bovine hemoglobin; ABM, artificial blood meal; FAC, ferric ammonium citrate; FER, ferritin purified from iron loaded pupae (Dunkov et al., 1995). The protein bands corresponding to LCH (28 kDa) and HCH (26 and 24 kDa) are indicated on the right.