Figure 4. Phase contrast micrographs of hemocytes incubated 18 h on Nunclon Delta 96-well tissue culture plates. 1.1 x 105 hemocytes/well. P indicates plasmatocytes; g indicates granular cell. (a) 0 µM zinc. (b) 100 µM zinc. (c) 2.5 µM phosphoramidon and 100 µM zinc. (d) 1 µM bestatin and 100 µM zinc. Note the greater order (more consistent number of plasmatocytes per node; more consistent distance between nodes) in the network formed in the presence of zinc than in the absence of zinc or in the presence of either phosphoramidon or bestatin. Arrows in images for no-zinc, phosphoramidon, and bestatin treatments point to areas where connections would be expected if the network were regularly organized. Bar = 100 µm.