Figure 3. Phase contrast micrographs of hemocytes incubated 18 h on Nunclon Delta 96-well tissue culture plates. (a) 5.5 x 104 cells/well. (b) 8.7 x 104 cell/well. (c) 1.1 x 105 cell/well. (d) 1.4 x 105 cell/well. P indicates plasmatocytes; g indicates granular cell. At the lowest concentration, plasmatocytes connected (arrow points to two connected plasmatocytes) but failed to form an elaborate network; granular cells remained dispersed apparently randomly. At the highest concentration, plasmatocytes were primarily present in distinct nodes often with granular cells on and around them (arrows point to nodes with accompanying granular cells). Bar = 100 µm.