Table 1 Aedes albopictus C7-10 cells, transfected with EcRE-2R-CAT constructs.

Input DNA (per plate) Recovery of stable transformats Time for recovery (days) 2R 2L AT0 2R 2L AT0 7 µg - + + -* 13 13 0.7 µg - + + - 13 13 70 ng + + + 16 13 13 7 ng + + + 14 13 13 0.7 ng + + + 14 13 13 0.07 ng + + + 14 12 12

Cells were stably transfected with plasmids EcRE-2R-CAT, EcRE-2L-CAT or ptkATO from L. Dobens (Dobens et al., 1991). The asterisk indicates that no clones were recovered by day 16.