Figure 1. Sequence of the DNA fragment containing two tandem EcREs from the Drosophila hsp27 gene in EcRE-2R-CAT. Motifs resembling mammalian AP-1 binding sites are shown in boxes. Note that two contiguous sites are shown in the 3'-terminal box. The two tandem direct repeats of the 23 bp EcRE element in the 2R (sense) orientation (Dobens et al., 1991) are shown in lower case. Primers (EcRE-1 and EcRE-2) used to generate a fragment (termed EcRE) from EcRE-2R-CAT are shown by arrows. The resultant fragment was digested with SalI (*S) and XhoI(**X)to generate a 206 bp fragment that was cloned into hsp-cat 1 (DiNocera and Dawid, 1983; see Figure 2) and also used as probe for southwestern blotting. The downward-pointing arrow (BamHI) indicates the 5'-end of the shorter 97 and 184 bp probes; the 3'-ends of these smaller probes are identified by HpaII (H) and BglII (Bg) sites, respectively.