Figure 3. Methoprene stimulated vitellogenin levels in allatectomized adult virgin females of Leucophaea maderae. Each female received three active prothoracic glands about two weeks prior to the application of methoprene in the doses indicated. Controls received the solvent only. Three days after methoprene treatment the animals were bled and the vitellogenin content of aliquots of the hemolymph was determined by rocket immunoelectrophoresis. Columns represent the averages SE of the mean of the number of animals indicated above the columns. ** denotes a P-value of < 1%o (student t-test).