Table 1 Predicted and determined biological activities (EC50 values) of relevant ecdysteroids in the ecdysteroid agonist version of the Drosophila melanogaster BII cell bioassay.

Predicated EC50 Determined EC50 Ecdysteroid (max nm#) Model A Model B 1: 20-hydroxyecdysone (242) 9M* 7.5x10- 2: ajugasterone C (243) 8M* 8.0x10- 3: muristerone A (236) 8M* 2.2x10- 4: turkesterone (244) 6M* 1.3x10- 5: dacryhainansterone (235 & 299) 9M 1.8x10-9M 5.6x10-11M 5.2x10- 6: kaladasterone (299) 7M 1.2x10-9M 2.6x10-10M 3.4x10- 7: 25-hydroxydacryhainansterone (299) 7M 3.0x10-9M 5.9x10-11M 2.6x10- 8: podecdysone B (244) 5M 4.6x10-11M 1.1x10-11M 1.2x10- 9: stachysterone B (298) 8M 2.0x10-9M 2.2x10-10M 8.2x10- 10: (14-H)7,9(11)-dien-6-one (296) 7M 2.2x10- 11: (14-H)7,9(11)-dien-6-one (298) 6M 1.3x10-

* Data taken from Dinan et. al., 1999 # in methanol