Figure 5. Effect of irradiation (350 nm for 20 min) in the presence of 100 nM 20-hydroxyecdysone (1) or dacryhainansterone (5) on the capacity of DmEcR/DmUSP (A) and CfEcR/CfUSP (B) ecdysteroid receptor complexes subsequently to bind [3H]ponasterone A; Filled bars, samples irradiated at 350 nm for 20 minutes; open bars samples not irradiated. Results shown are the mean values of duplicate samples; variation between replicates is 5% or less.