Figure 7. Immunodetection of DlEPV proteins using a mouse polyclonal anti-DlEPV serum generated against three (41-45, 48-50, and 53- 55 % sucrose) combined fractions of sucrose- purified virions and rabbit anti-mouse second antibody. At least six major viral protein bands are detected. Pre-immunized mouse serum (lane not shown) did not react with the antibody. MW=mass in kiloDaltons (kD), 25 and 50 kD markers=purified mouse IgG.