Figure 6. Dot blot of total DNA from male (A) and female (B) D. longicaudata and salmon sperm (C) (control) probed with DIG-labeled DlEPV DNA. Top row: 2µg DNA/lane; bottom row: 1µg DNA/ lane; D=DlEPV probe dilutions in descending concentrations 1, 0.5, 0.25, 0.12, and 0.06 µg DNA. The detection of DlEPV DNA in male and female wasps suggests that viral DNA occurs in males and also confirms earlier reports (Lawrence and Akin, 1990) that the virus occurs in female wasps.