Figure 3. Pulse field gel electrophoresis of DlEPV genomic DNA (D). Virions were sucrose-purified, digested in 1% pulse field grade (PFG) agarose plugs (BioRad) with proteinase K, as described in Materials an Methods, and run into a 1% PFG agarose gel in a CHEF DR II (BioRad) system in 0.5 x TBE at 14°C. The run time was 22h at 6V/cm with initial and final switch times of 60 and 90 sec., respectively, at an angle of 120°. The gel was stained with 0.25 mg/ml EtBr. The DlEPV genomic DNA is 290-300 kb (arrowhead). λ =Lambda concatemer of 50 Kb bands. Sc=Saccharomyces cerevisiae DNA.