Figure 4. Protein concentrations (means SEM) in the hemolymph of A) queen and B) worker pupae of Apis mellifera treated with either 10 mg of juvenile hormone (in 2 ml acetone), or with 2 ml of acetone (solvent control) during the pink-eyed pupal phase (Pp). n=3 (hemolymph pools). C) Hemolymph protein concentrations in worker pupae of Apis mellifera injected with 5 mg of ecdysone diluted in ethanol/Ringer's solution 1:4 (treated), or with ethanol/Ringer's solution (controls) during the brown-eyed pupal phase (Pbl). n=3 (hemolymph pools); Statistical analysis: Pbm II and Pbd-ecdysone treated differ from controls, p <0.05 (Mann-Whitney test). Pb: brown-eyed pupae with unpigmented cuticle; Pbl, Pbm and Pbd: brown-eyed pupae showing light, intermediary or dark pigmented cuticle, respectively.