Figure 1. Vitellogenin titers in hemolymph of pupal stages and young adults of Apis mellifera queens (A) and workers (B). Rocket immunoelectrophoreses with 3mg hemolymph proteins per well were carried out in 1% agarose gels containing 1% rabbit antiserum raised against honeybee egg proteins. Pw: white-eyed pupae; Pp: pink-eyed pupae; Pb: brown-eyed pupae, all of them with still unpigmented cuticle; Pbl: brown eyed pupae initiating cuticular pigmentation; Pbm: brown eyed pupae showing intermediary grade of cuticular pigmentation; Pbd: brown eyed pupae showing dark pigmented cuticle; Pha: pharate adult; Ne: newly emerged bee; 1d-3d: days after eclosion.