Oosorption in the endoparasitoid, Pteromalus puparum
1State Key Laboratory of Rice Biology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop
Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310029, China
2Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Science, China
Jiliang University, Hangzhou 310018, China
Abstract
Oosorption is the resorption of oocytes in the ovaries, and is usually induced by environmental stress. It has been demonstrated in some insect species, but overall the mechanisms of oosorption are poorly understood. In this study, the oosorption in the endoparasitic wasp, Pteromalus puparum L. (Hymenoptera: Pteromalidae), was observed in response to starvation. To explore the details of oosorption in P. puparum, both levels of hemolymph vitellogenin and ovarian vitellin were determined using sandwich ELISA. The results indicated that both levels of vitellin and total protein in the ovaries were significantly decreased 48 h after eclosion in starved P. puparum, while those of vitellogenin and total protein in the hemolymph were increased. In addition, observation of the ultrastructure of mature oocytes in the ovarioles revealed changes in yolk protein content. Those protein yolk spheres and lipid yolk spheres that had accumulated in the oocytes, were transferred out of the oocytes of starved females. It was assumed that once oosorption was induced in P. puparum, vitellin in the oocytes was transported outside and released into the hemolymph. This information helps to elucidate a mechanism of oosorption in insects.
Keywords: oocyte, starvation, ultrastructure, vitellin, vitellogenin
Correspondence:
a gjy107@163.com,
b dong_shzhang@163.com,
c chu@zju.edu.cn,
d likai@dhu.edu.cn,
e jiayingzhu001@yahoo.com.cn,
f giggs0574@sina.com,
g gyye@zju.edu.cn, *Corresponding author
Received: 11 July 2010 | Accepted: 13 January 2011 | Published: 17 July 2011
Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
ISSN: 1536-2442 | Volume 11, Number 90
Guo J-y, Dong S-z, Ye G-y, Li K, Zhu J-y, Fang Q, Hu C. 2011. Oosorption in the endoparasitoid, Pteromalus puparum. Journal of Insect Science 11:90 available online: insectscience.org/11.90
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