The putative AKH Receptor of the tobacco hornworm, Manduca sexta, and its expression
1Department of Entomology, The University of Arizona, Tucson, AZ 85721 USA
2Department of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ 85721 USA
Abstract
Adipokinetic hormones are peptide hormones that mobilize lipids and/or carbohydrates for flight in adult insects and activate glycogen phosphorylase in larvae during starvation and during molt. We previously examined the functional roles of adipokinetic hormone in Manduca sexta L. (Lepidoptera: Sphingidae). Here we report the cloning of the full-length cDNA encoding the putative adipokinetic hormone receptor from the fat body of M. sexta. The sequence analysis shows that the deduced amino acid sequence shares common motifs of G protein-coupled receptors, by having seven hydrophobic transmembrane segments. We examined the mRNA expression pattern of the adipokinetic hormone receptor by quantitative Real-Time PCR in fat body during development and in different tissues and found the strongest expression in fat body of larvae two days after molt to the fifth instar. We discuss these results in relation to some of our earlier results. We also compare the M. sexta adipokinetic hormone receptor with the known adipokinetic hormone receptors of other insects and with gonadotropin releasing hormone-like receptors of invertebrates.
Keywords: Adipokinetic hormone, adipokinetic hormone receptor, fat body, mRNA expression, Real Time PCR
Abbreviations: ACP, AKH/corazonin-related peptide; AKH, adipokinetic hormone; CC, corpora cardiaca; GP, glycogen phosphorylase; qRT-PCR, Real Time PCR; RPCH, red pigment concentrating hormone
Correspondence:
a* rolf@email.arizona.edu,
b aedesrock@gmail.com,
c wmoore@email.arizona.edu,
d mriehle@ag.arizona.edu, *Corresponding author
Received: 23 February 2010 | Accepted: 10 August 2010 | Published: 5 April 2011
Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
ISSN: 1536-2442 | Volume 11, Number 40
Ziegler R, Isoe J, Moore W, Riehle MA, Wells MA. 2011. The putative AKH Receptor of the tobacco hornworm, Manduca sexta, and its expression. Journal of Insect Science 11:40 available online: insectscience.org/11.40
 Figure 1 |
 Figure 2 |
 Figure 3 |
 Figure 4 |
 Figure 5 |
 Figure 6 |
 Figure 7 |
 Figure 8 |