Molecular phylogeny and identification of the peach fruit fly, Bactrocera zonata, established in Egypt
1 Entomology Department, Faculty of Science, Cairo University, Giza 12613, Egypt
2 Genetics Department, Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt
Abstract
The genetic structure of the Egyptian peach fruit fly (Bactrocera zonata (Saunders) (Diptera: Tephritidae)) population was analyzed using total RNA from adult females. A portion of mitochondrial cytochrome oxidase I (COI), 369 bp was amplified using RT-PCR, and was sequenced and analyzed to clarify the phylogenetic relationship of B. zonata established in Egypt. The data suggested that the gene shared a similarity in sequence compared to Bactrocera COI gene found in GenBank. Molecular phylogenetic analyses were performed based on nucleotide sequences in order to examine the position of the Egyptian population among many other species of fruit flies. The results indicate that four accession numbers of B. zonata (three from New Zealand and one from India) are closely related, while the Egyptian B. zonata are close to the 71 accession numbers of Bactrocera include one B. zonata from New Zealand. These two B. zonata from Egypt and New Zealand showed a close relationship in neighbor–joining analysis using the seven accession numbers of B. zonata. In addition, a theoretical restriction map of the homology portion of the COI gene was constructed using 212 restriction enzymes obtained from the restriction enzyme database to identify the Egyptian and New Zealand B. zonata.
Keywords: mitochondrial cytochrome oxidase I gene (COI), restriction map, RT-PCR
Abbreviations: PCR, polymerase chain reaction; RT, reverse transcription; RFLP, restriction fragment length polymorphism
Correspondence:
a dr_emyali@hotmail.com,
b zakielfiky@yahoo.com, *Corresponding author
Editor: Craig Coates was Editor of this paper.
Received: 10 June 2010 | Accepted: 14 October 2011 | Published: 31 December 2011
Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
ISSN: 1536-2442 | Volume 11, Number 177
Abd-El-Samie EM, El Fiky ZA. 2011. Molecular phylogeny and identification of the peach fruit fly, Bactrocera zonata, established in Egypt. Journal of Insect Science 11:177 available online: insectscience.org/11.177
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