Download free PDF

Abstract

Southern chinch bug, Blissus insularis Barber (Heteroptera: Blissidae), is the most destructive insect pest of St. Augustine grass, Stenotaphrum secundatum Waltz (Kuntze), in the southern United States. The present study is focused on assessing genetic variability in five populations of B. insularis collected from Texas and Florida where St. Augustine grass is widely grown. The amplified fragment length polymorphism technique was used to DNA fingerprint individuals from each population (a total of 46 individuals) using five primer combinations (EcoRI/MSeI). Analysis of molecular variance results show no evidence to support significant genetic variability among Texas and Florida populations of B. insularis. Nearly all genetic variation was found to reside within populations (95%), with only approximately 3% residing among populations between regions. Low G_ST values obtained from POPGENE and low F_ST values obtained from the analysis of molecular variance both support the conclusion for high levels of gene flow resulting from interbreeding and/or migratory events among the populations. A Mantel test of Euclidean squared distances showed no correlation between the genetic distance and geographic distance matrices of tested populations of B. insularis. The results of the present study suggests that gene flow is occurring among populations of B. insularis and, therefore, breeders need to be aware that this resistance will most likely not remain localized, and it has the potential to spread as a result of migratory events.

Keywords: AMOVA, gene flow, genetic distance, St. Augustine grass

Abbreviations: AFLP, amplified fragment length polymorphism; AMOVA, analysis of molecular variance; F_ST, genetic divergence; G_ST, total gene diversity estimates; Nm, gene flow estimates; PCoA, principle component analysis

Correspondence: ^a* a-chandra@tamu.edu, ^b j-reinert@tamu.edu, ^c j-lamantia@tamu.edu, ^d blakepond@gmail.com, ^e drh15@psu.edu, *Corresponding author

Received: 22 June 2010 | Accepted: 6 January 2011 | Published: 31 December 2011

Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.  

ISSN: 1536-2442 | Volume 11, Number 173

Chandra A, Reinert JA, LaMantia J, Pond JB, Huff DR. 2011. Genetic variability in populations of the southern chinch bug, Blissus insularis, assessed using AFLP analysis. Journal of Insect Science 11:173 available online: insectscience.org/11.173