Mining and characterization of sequence tagged microsatellites from the brown planthopper Nilaparvata lugens
Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China
Abstract
The brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is an important pest of rice. To better understand the migration pattern and population structure of the Chinese populations of N. lugens, we developed and characterized 12 polymorphic microsatellites from the expressed sequence tags database of N. lugens. The occurrence of these simple sequence repeats was assessed in three populations collected from three provinces of China. The number of alleles per locus ranged from 3 to 13 with an average of 6.5 alleles per locus. The mean observed heterozygosity of the three populations ranged from 0.051 to 0.772 and the expected heterozygosity ranged from 0.074 to 0.766. The sequences of the 12 markers were highly variable. The polymorphism information content of the 12 markers was high and ranged from 0.074 to 0.807 (mean = 0.503). Sequencing of microsatellite alleles revealed that the fragment length differences were mainly due to the variation of the repeat motif. Significant genetic differentiation was detected among the three N. lugens populations as the Fst ranged from 0.034 to 0.273. Principle coordinates analysis also revealed significant genetic differentiation between populations of different years. We conclude that these microsatellite markers will be a powerful tools to study the migration routine of the N. lugens.
Keywords: migration routine, expressed sequences, population genetics
Abbreviations: EST, expressed sequence tag; GX, Guangxi province, JS, Jiangsu province; SSR, simple sequence repeats; ZJ, Zhejiang province
Correspondence:
a 2009202023@njau.edu.cn,
b kjzhangcn@gmail.com,
c mee@njau.edu.cn,
d xyhong@njau.edu.cn, *Corresponding author
Editor: Igor Sharakhov was Editor of this paper.
Received: 31 October 2010 | Accepted: 20 May 2011 | Published: 4 October 2011
Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
ISSN: 1536-2442 | Volume 11, Number 134
Sun J-T, Zhang Y-K, Ge C, Hong X-Y. 2011. Mining and characterization of sequence tagged microsatellites from the brown planthopper Nilaparvata lugens. Journal of Insect Science 11:134 available online: insectscience.org/11.134
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