Phylogenetic analysis and rapid identification of the whitefly, Bemisia afer, in China
1High-tech Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China
2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection (South Campus),
Chinese Academy of Agricultural Sciences, Beijing, 100081, China
3Plant Pest Diagnostic Center, 3294 Meadowview Road, Sacramento, California, 95832 USA
Abstract
The phylogenetic relationship between the whitefly Bemisia afer (Priesner & Hosny) (Hemiptera: Aleyrodidae) from China and other populations among the world were analyzed based on the mitochondrial cytochrome oxidase I (mtCOI) gene. Phylogenetic analysis of mtCOI sequences and those of reference B. afer sequences showed that the populations of the species could be separated into 5 clades (I-V). There were at least two clades of the species from China (IV and V). These data suggested that B. afer might be a species complex. The Chinese B. afer populations were most divergent with B. afer from the United Kingdom and African countries. The distance between the Chinese B. afer (IV and V) and clades I, II, and III is more than 32%, while the distance among clades I, II, III is lower than 7.7%. A new set of primers specific to B. afer was designed to amplify a region of approximately 400 bp to discriminate B. afer from other Bemisia species in China based on mtCOI sequences.
Keywords: mitochondrial cytochrome oxidase I, molecular markers
Abbreviation: mtCOI, mitochondrial cytochrome oxidase I gene
Correspondence: achudong1977@hotmail.com, brgill@cdfa.ca.gov
Received: 2 July 2008 | Accepted: 24 November 2008 | Published: 1 July 2010
Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
ISSN: 1536-2442 | Volume 10, Number 86
Chu D, Liu G, Wan F, Tao Y, Gill RJ. 2010. Phylogenetic analysis and rapid identification of the whitefly, Bemisia afer, in China. Journal of Insect Science 10:86, available online: insectscience.org/10.86
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