Study of oostatic peptide uptake and metabolism in developing ovaries of the flesh fly, Neobellieria bullata

Abstract

The uptake and metabolism of the oostatic pentapeptide analogue of trypsin modulating oostatic factor (TMOF), H-Tyr-Asp-Pro-Ala-Pro-OH (5P), in ovaries of Neobellieria bullata (Parker) (Diptera: Sarcophagidae) were analyzed during their developmental stages. During selected stages of yolk deposition, the fate of [³HPro³]5P after its in vivo injection was compared to its uptake after in vitro incubation of dissected ovaries. The ovaries were analyzed from 30 s to 180 min after incubation. A detection sensitivity of 60-100 fmol of the labeled 5P was achieved using radio-high performance liquid chromatography. While the uptake of the applied radioactivity strongly depended on the stage of vitellogenesis, especially for the in vitro experiment, degradation of 5P was very quick and independent of whether the label was injected or incubated with the ovaries, regardless of the developmental stage of ovaries. No tracers of 5P were detected at 30 s after applying the labeled 5P in all tests.

Keywords: Diptera, Sarcophagidae, follicular cells, oostatic peptide, in vivo uptake, in vitro uptake, metabolites
Abbreviations: HPLC, high performance liquid chromatography; TMOF, trypsin modulating oostatic factor

Correspondence: ^*honzah@uochb.cas.cz

Received: 11 July 2008 | Accepted: 23 June 2009 | Published: 17 May 2010

Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.  

ISSN: 1536-2442 | Volume 10, Number 48

Bennettová B, Slaninová J, Vlasáková V, Hlaváček J, Holík J and Tykva R. 2010. Study of oostatic peptide uptake and metabolism in developing ovaries of the flesh fly, Neobellieria bullata. Journal of Insect Science 10:48, available online: insectscience.org/10.48