Differential gene expression from midguts of refractory and susceptible lines of the mosquito, Aedes aegypti, infected with Dengue-2 virus
1 Centro Internacional de Entrenamiento e Investigaciones Médicas, Cali, Colombia
2 Department of Biological Sciences, Simon Fraser University, Burnaby B.C., Canada
Abstract
Suppressive subtractive hybridization was used to evaluate the differential expression of midgut genes of feral populations of Aedes aegypti (Diptera: Culicidae) from Colombia that are naturally refractory or susceptible to Dengue-2 virus infection. A total of 165 differentially expressed sequence tags (ESTs) were identified in the subtracted libraries. The analysis showed a higher number of differentially expressed genes in the susceptible Ae. aegypti individuals than the refractory mosquitoes. The functional annotation of ESTs revealed a broad response in the susceptible library that included immune molecules, metabolic molecules and transcription factors. In the refractory strain, there was the presence of a trypsin inhibitor gene, which could play a role in the infection. These results serve as a template for more detailed studies aiming to characterize the genetic components of refractoriness, which in turn can be used to devise new approaches to combat transmission of dengue fever.
Keywords: suppressive subtractive hybridization
Abbreviations: EST, expressed sequence tag; SSH, suppressive subtractive hybridization
Correspondence: aclaraocampo@cideim.org.co
Associate Editor: Jan Veenstra was editor of this paper
Received: 13 February 2008 | Accepted: 1 September 2008 | Published: 8 May 2010
Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
ISSN: 1536-2442 | Volume 10, Number 41
Barón OL, Ursic-Bedoya RJ, Lowenberger CA, Ocampo CB. 2010. Differential gene expression from midguts of refractory and susceptible lines of the mosquito, Aedes aegypti, infected with Dengue-2 virus. 23pp. Journal of Insect Science 10:41, available online: insectscience.org/10.41
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