Synergistic and antagonistic effects of piperonyl butoxide in fipronil-susceptible and resistant rice stem borrers, Chilo suppressalis
Shanghai Key laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, China
Abstract
Using the phenylpyrazole insecticide, fipronil for selection in the laboratory, a resistant Wenzhou strain of the rice stem borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae) had an LD50 at least 45.3 times greater than the susceptible Anhui strain. The realized resistant heritability (h2) of 0.213 showed that the tolerant phenotype was moderately heritable and had potential to develop higher tolerance to fipronil. Piperonyl butoxide decreased the effects of fipronil on the mortality of the susceptible larvae with 0.27-0.44 times synergistic rates, but increased the toxicity of fipronil on the resistant larvae with 1.85-2.53 times synergistic rates as compared to that of fipronil alone. The inhibitory effect of piperonyl butoxide on the activity of microsomal O-demethylase was greater in susceptible larvae than in the resistant larvae. The differential synergism of fipronil by piperonyl butoxide in the susceptible and resistant C. suppressalis may be caused by the reduced penetration of fipronil in the lab-selected Wenzhou strain.
Keywords: synergism, resistance, phenylpyrazole insecticide
Abbreviations: PBO, piperonyl butoxide; MOD, microsomal O-demethylase; PMSF, phenylmethyl sulfonyl
fluoride; NADPH, β-nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt; PTU, phenylthiourea;
EDTA, ethylenediamine tetraacetic acid; DTT, 1,4-dithiothreitol; p-NA, p-nitroanisole
Correspondence:
*qchuang@ecust.edu.cn
Associate Editor: James Ottea was editor of this paper
Received: 21 May 2009 | Accepted: 11 January 2010 | Published: 21 October 2010
Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
ISSN: 1536-2442 | Volume 10, Number 182
Huang Q, Deng Y, Zhan T, He Y. 2010. Synergistic and antagonistic effects of piperonyl butoxide in fipronil-susceptible and resistant rice stem borrers, Chilo suppressalis. Journal of Insect Science 10:182, available online: insectscience.org/10.182
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