Amplified fragment length polymorphism mapping of quantitative trait loci for economically important traits in the silkworm, Bombyx mori
1Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, PO Box 41635-1314, Rasht,
Guilan, Iran
2Department of Agronomy & Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, PO Box 41635-
1314, Rasht, Guilan, Iran
3Department of Genomics, Agricultural Biotechnology Research Institute of Iran (Rasht), PO Box 41635-4115,
Rasht, Guilan, Iran
4Department of Sericulture, Faculty of Agricultural Sciences, University of Guilan, PO Box 41635-1314, Rasht,
Guilan, Iran
Abstract
Cocoon related characteristics are economically important traits in the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae). In this study a genetic linkage map was developed that identified QTL controlling the cocoon weight, cocoon shell weight, and cocoon shell percentage using 161 amplified fragment length polymorphism (AFLP) markers. Twenty PstI/TaqI primer combinations were employed to genotype 78 F2 progenies derived from a cross between P107 Japanese inbred line and Khorasan Lemon Iranian native strain. Among polymorphic markers, 159 AFLP markers were assigned to 24 linkage groups at the LOD threshold of 2.5 that varied in length from 4 to 299 cM. The total length of the linkage map was 2747 cM, giving an average marker resolution of 19.31 cM. A total of 21 AFLP markers were identified that were distributed over the ten linkage groups linked to the three studied traits using the composite interval mapping method. The explained variation rate by QTL controlling cocoon weight, cocoon shell weight, and cocoon shell percentage ranged from 0.02% to 64.85%, 0.2% to 49.11%, and 0.04% to 84.20%, respectively. These QTL controlled by different actions as well as under dominance, additive, partial dominance, dominance, and over dominance.
Keywords: AFLP markers, cocoon traits, QTL mapping
Abbreviations: ABRII, Agricultural Biotechnology Research Institute of Iran; AFLP, Amplified Fragment Length
Polymorphism; AREO, Agricultural Research and Education Organization; BC, Backcross; CIM, Composite Interval
Mapping; cM, centimorgans; ESTs, Expressed Sequence Tags; IM, Interval Mapping; ISRC, Iran Silkworm Research
Center; LG, Linkage Group; LOD, Logarithm of Odds; LRT, Likelihood Ratio Test; NPV, Nuclear Polyhedrosis Virus;
PCR, Polymerase Chain Reaction; QTL, Quantitative Trait Loci; RAPD, Random Amplified Polymorphic DNA; RFLP,
Restriction Fragment Length Polymorphism; SADF, Selective Amplification of DNA Fragments; SNP, Single Nucleotide
Polymorphism
Correspondence:
amirhosin@guilan.ac.ir,
brabiei@guilan.ac.ir,
cpayam_572003@abrii.ac.ir,
d*bendalir@guilan.ac.ir, *Corresponding author
Associate Editor: Craig Coates was editor of this paper.
Received: 7 April 2009 | Accepted: 2 May 2010 | Published: 14 September 2010
Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
ISSN: 1536-2442 | Volume 10, Number 153
Mirhoseini SZ, Rabiei B, Potki P, Dalirsefat SB. 2010. Amplified fragment length polymorphism mapping of quantitative trait loci for economically important traits in the silkworm, Bombyx mori. Journal of Insect Science 10:153, available online: insectscience.org/10.153
 Figure 1 |
 Figures 2 |
 Figure 3 |
 Figure 4 |