Identification and expression analysis of minichromosome maintenance proteins in the silkworm, Bombyx mori
Laboratories of Insect Genetic Resources, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan
Abstract
The minichromosome maintenance protein (MCM) family is involved in the regulatory role of DNA replication in eukaryotic organisms. A cDNA encoding of an MCM of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequenced. The resultant amino acid sequence and phylogenetic analysis revealed high identity to MCM, and specifically to MCM7, of vertebrates and invertebrates. An RT-PCR showed that the bmMCM7 transcript was present in the ovaries, testes, silk glands, and fat bodies of larval silkworms. Expression plasmids were transformed into competent Escherichia coli and overexpressed. This is the first report on the identification of MCM helicase of the silkworm, B. mori.
Keywords: DNA replication, helicase
Abbreviation: ARS, autonomous replication sequences; IPTG, isopropyl 1-thio-ß-D-galactoside; MCM, a
minichromosome maintenance protein; RT-PCR, reverse transcriptase-polymerase chain reaction
Correspondence: *yamamok@agr.kyushu-u.ac.jp
Received: 4 February 2009 | Accepted: 21 February 2010 | Published: 10 September 2010
Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
ISSN: 1536-2442 | Volume 10, Number 148
Yamamoto K, Aso Y, Fujii H. 2010. Identification and expression analysis of minichromosome maintenance proteins in the silkworm, Bombyx mori. Journal of Insect Science 10:148, available online: insectscience.org/10.148
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