Observation of the peach fruit moth, Carposina sasakii, larvae in young apple fruit by dedicated micro-magnetic resonance imaging

Mika Koizumi1a, Fumio Ihara2b, Katsuhiko Yaginuma2c, Hiromi Kano3d, and Tomoyuki Haishi4e

1Research Institute for Science and Engineering, Waseda University, 2-2 Wakamatsu-cho, Shinjyuku, Tokyo 162- 8480, Japan
2National Institute of Fruit Tree Science, National Agriculture and Food Research Organization, 2-1 Fujimoto, Tsukuba, Ibaraki 305-8605, Japan
3Oak-Hill Georgic Patch-Work Laboratory, 4-13-10 Miyamoto, Funabashi, Chiba 273-0003, Japan
4MRTechnology, Inc., 2-1-6 Sengen, Tsukuba, Ibaraki 305-0047, Japan

Abstract

Infestation of young apple fruits by the larvae of the peach fruit moth, Carposina sasakii Matsumura (Lepidoptera: Carposinidae), was studied by a small dedicated micro-magnetic resonance imaging (MRI) apparatus using the three-dimensional (3D) gradient-echo method and the two-dimensional (2D) and 3D spin-echo methods. Changes from a young larva at 1.8 mm in length to a mature one ready to leave the fruit were observed in relation to the progression of infestation of the fruit tissues. The trace of larva intrusion was demonstrated by a series of sliced images in the 3D image data of an infested fruit, where it entered from outside the calyx, and migrated to near the vasculature around the carpel through the core. The small, dedicated MRI device was proven useful for ecological studies of the growth and movement of insect larvae in their food fruits. It can also be applied to detect the infestation of small fruits by insect larvae.

Keywords: growth, infestation, movement, small MRI apparatus with a permanent magnet
Abbreviation: MRI, magnetic resonance imaging; TR, repetition time; TE, echo time; 2D, two-dimensional; 3D, three-dimensional

Correspondence: am.koizumi4@kurenai.waseda.jp, bihara@affrc.go.jp, cyaginuma@affrc.go.jp, dtoradaiou@gamma.ocn.ne.jp, ehaishi@mrtechnology.co.jp, *Corresponding author
Associate Editor: Tugrul Giray was editor of this paper.

Received: 25 December 2008 | Accepted: 3 October 2009 | Published: 10 September 2010

ISSN: 1456-2442 | Volume 10, Number 145

Koizumi M, Ihara F, Yaginuma K, Kano H, Haishi T. 2010. Observation of the peach fruit moth, Carposina sasakii, larvae in young apple fruit by dedicated micro-magnetic resonance imaging. Journal of Insect Science 10:145, available online: insectscience.org/10.145


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