Molecular cloning, expression profile and 5’ regulatory region analysis of two chemosensory protein genes from the diamondback moth, Plutella xylostella
Key Laboratory of Pesticide and Chemical Biology, Ministry of Education of P.R. China. South China Agricultural University, Guang Zhou, 510642, Guangdong, China
Abstract
Chemosensory proteins play an important role in transporting chemical compounds to their receptors on dendrite membranes. In this study, two full-length cDNA codings for chemosensory proteins of Plutella xylostella (Lepidoptera: Plutellidae) were obtained by RACE-PCR. Pxyl- CSP3 and Pxyl-CSP4, with GenBank accession numbers ABM92663 and ABM92664, respectively, were cloned and sequenced. The gene sequences both consisted of three exons and two introns. RT-PCR analysis showed that Pxyl-CSP3 and Pxyl-CSP4 had different expression patterns in the examined developmental stages, but were expressed in all larval stages. Phylogenetic analysis indicated that lepidopteran insects consist of three branches, and Pxyl- CSP3 and Pxyl-CSP4 belong to different branches. The 5’regulatory regions of Pxyl-CSP3 and Pxyl-CSP4 were isolated and analyzed, and the results consist of not only the core promoter sequences (TATA-box), but also several transcriptional elements (BR-C Z4, Hb, Dfd, CF2-II, etc.). This study provides clues to better understanding the various physiological functions of CSPs in P. xylostella and other insects.
Keywords: cDNA cloning, genomic structure, transcription factor recognition site analysis
Abbreviation: BR-C Z4,Broad-Complex Z4; CSP, Chemosensory protein; CF2-II, Zinc finger domain; Dfd,
Deformed; Hb, Hunchback; ORF, open reading frames; Pxyl, Plutella xylostella; RACE, rapid amplification of cDNA
ends; UTR, untranslated region
Correspondence:
a125314689@qq.com,
bguohuazhong@scau.edu.cn
c*humy@scau.edu.cn,
d13929346@qq.com,
e542438779@qq.com, *Corresponding author
Associate Editor: Zhijian Jake Tu was editor of this paper.
Received: 14 December 2008 | Accepted: 9 June 2009 | Published: 10 September 2010
Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
ISSN: 1536-2442 | Volume 10, Number 143
Gong L, Zhong GH, Hu MY, Luo Q, Ren ZZ. 2010. Molecular cloning, expression profile and 5’ regulatory region analysis of two chemosensory protein genes from the diamondback moth, Plutella xylostella. Journal of Insect Science 10:143, available online: insectscience.org/10.143
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