Isolation of microsatellite markers in the Calliptamus genus (Orthoptera, Acrididae)
1CIRAD, UPR Acridologie, Montpellier, F-34398 France
2CIRAD, UMR Development and Adaptation of Plants, Montpellier, F-34398 France
Abstract
The Calliptamus genus (Orthoptera: Acrididae) includes locust and grasshopper species, some of which have a high economic impact. Using an enriched methodology, 10 microsatellite markers have been developed from two species, Calliptamus italicus and Calliptamus barbarus. These polymorphic markers were tested on different populations of three Calliptamus species: C. italicus, C. barbarus, C. wattenwylianus. Two markers were amplified on the three species, as well as four on C. barbarus and two on C. italicus. In each species, 9 to 23 alleles per locus were observed. These molecular markers might prove to be a new and interesting tool for Calliptamus population genetics and dispersion studies.
Keywords: Orthoptera, Calliptaminae, polymorphic
Correspondence:
a*blanchet.elodie@gmail.com,
bchristine.pages@cirad.fr
claurence.blondin@cirad.fr,
dclaire.billot@cirad.fr,
eronan.rivallan@cirad.fr,
fJean-Michel.Vassal@cirad.fr,
gmichel.lecoq@cirad.fr, hristerucci@cirad.fr, *Corresponding author
Associate Editor: Craig Coates was editor of this paper.
Received: 16 December 2008 | Accepted: 29 January 2009 | Published: 13 August 2010
Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
ISSN: 1536-2442 | Volume 10, Number 133
Blanchet E, Pages C, Blondin L, Billot C, Rivallan R, Vassal JM, Lecoq M, Risterucci AM. 2010. Isolation of microsatellite markers in the Calliptamus genus (Orthoptera, Acrididae) Journal of Insect Science 10:133, available online: insectscience.org/10.133