Effect of induced oxidative stress and herbal extracts on acid phosphatase activity in lysosomal and microsomal fractions of midgut tissue of the silkworm, Bombyx mori
1Department of Zoology, Shivaji University, Kolhapur, Maharashtra State, India
2Department of Zoology, Bhogawati College, Kurukali, Karveer, Kolhapur, Maharashtra State, India
Abstract
Lysosomal and microsomal acid phosphatase activity was estimated in midgut tissue of silkworm larvae, Bombyx mori L. (Lepidoptera: Bombycidae), after induced oxidative stress by Dgalactose. The larvae were simultaneously treated with ethanolic extracts of Bacopa monniera and Lactuca sativa to study their antioxidant properties. Lipid peroxidation and fluorescence was measured to analyze extent of oxidative stress. The ethanolic extract of Lactuca sativa was found to be more effective in protecting membranes against oxidative stress than Bacopa monniera.
Keywords: D-galactose, Bacopa monniera and Lactuca sativa
Abbreviations: ACP, acid phosphatase; ROS, reactive oxgygen species
Correspondence:
agaikwad347@rediffmail.com
Received: 11 November 2008 | Accepted: 18 July 2009 | Published: 16 July 2010
Copyright: This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed.
ISSN: 1536-2442 | Volume 10, Number 113
Gaikwad YB, Gaikwad SM, Bhawane GP. 2010. Effect of induced oxidative stress and herbal extracts on acid phosphatase activity in lysosomal and microsomal fractions of midgut tissue of the silkworm, Bombyx mori. Journal of Insect Science 10:113, available online: insectscience.org/10.113
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