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Table 3 Comparison between aqueous and ethanol biolistic conditions.
Shooting
conditions		 µg DNA/
mg of
particles 		Number
of
shootings 		Number
of positive
shootings 		Embryo
number per
shooting 		Number
of shot
embryos 		Number
of
positive
embryos 		Number
blue
spots 		Mean
number of
blue spots
per
embryo 		Percentage
of positive
embryos
UNS conditions
55-Ø7 Ø10 nozzle**
1800 psi, aqueous 		4/0.5 5 5 6-7 31 23 137 5.95 74
UNS conditions
55-Ø7 Ø10 nozzle
1800 psi, ethanol		 4/0.5 5 5 5-6 28 28 >441* >15.75 100
The vector used was pBRJZ
* For two embryos in one shooting, the amount of staining was too high to be counted because of overlapping.
** The numbers describe the nozzle dimensions. See the results.
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