Back One Page
Table 1 Biolistic results : effect of the use of a focus nozzle in Bombyx mori embryos
Shooting
conditions		 µg DNA/
mg of
particles 		Number
of
shootings 		Number
of positive
shootings 		Embryo
number per
shooting 		Number
of shot
embryos 		Number
of
positive
embryos 		Number
blue
spots 		Mean
number of
blue spots
per
embryo 		Percentage
of positive
embryos
Bio Rad
1800 psi 		2/0.5 9 5 10 90 8 18* 0.2 8.8
Bio Rad
1100 psi 		2/0.5 4 3 4 16 9 10* 0.625 56
Brass nozzle 17-Ø
7 floppy
macrocarrier 1800
psi 		2/0.5 3 3 10-10-4 24 12 48** 2 50
Brass nozzle 17-Ø
7 rigid
macrocarrier 1800
psi 		2/0.5 5 5 6-8 36 24 590*** 16.38 66.6
The vector used was pBRJZ
For experiments 1 and 2 the distance between the stopping screen and the target area was 101 mm. Nearer was not possible because of the strength of the blast effect.
For experiment 3, the distance from the nozzle tip and the target area was 80 mm.
For experiment 4, the distance between the tip of the nozzle and the target area was 30 mm. With a distance of 1 cm the blast effect is too strong and blows out the embryos. With a distance of 20 mm effect was intermediate but not so good as with a distance of 30 mm (data not shown). The reduced distance of experiment 3 was possible due to the use of rigid macrocarrier and the associated valve effect.
*All staining was superficial on the integument.
**Staining was superficial and internal
***Almost all staining was internal
Back One Page