Table 1 Intersimple sequence repeat (ISSRs) markers in different taxa, by taxon name, UBC primer number, sequence, number of bands amplified, number of polymorphic bands, and maximal sequence divergence between samples (uncorrected p).

Taxon Species Collection locations UBC Primer no. (5'-3') Sequence (5'-3') No. of bands amplifieda,c No. of polymorphic bandsb (%) Max sequence divergence (%; region, size) Aphid Pemphigus obesinymphae Santa Cruz Co., Arizona 809 811 812 819 826 827 841 857 (AG)8G (GA)8C (GA)8A (GT)8A (AC)8C (AC)8G (GA)8YC (AC)8YC 8 9 5 5 9 8 1 4 3 (38%) 2 (22%) 1 (20%) 0 2 (22%) 6 (75%) 0 0 0.00% (COI/II, 1400 bp)1 Acyrthosiphon pisum Dane Co, Wisconsin; Pima Co., Az. 809 811 827 (AG)8G (GA)8C (AC)8G 5 10 5 2 (40%) 3 (30%) 2 (40%) 0.14% (TrpE/G Buchnera plasmid)2 Mosquito Aedes aegypti Pima Co., Az.; Bexar Co., Texas 811 818 827 (GA)8C (CA)8G (AC)8G 6 7 6 2 (33%) 5 (71%) 0 1.3% (COI, 791 bp)3 Rotifer Philodina spp. El Paso Co., Tx; Santa Cruz Co., Az.; Yuma Co., Az. 808 826 827 868 (AG)8C (AC)8C (AC)8G (GAA)6 12 12 5 9 10 (83%) 6 (50%) 2 (40%) 2 (22%) 13% (COI, 591 bp)4

a The cumulative number of bands distinguishable across different genotypes from the given localities. Weak and smeared bands were not scored. b The approximate number of distinguishable polymorphic bands, given that weak or smeared bands were not scored. c Primers also screened but which gave weak or no interpretable bands were: P. obesinymphae, UBC nos. 801-8, 810, 813-8, 820-5, 828-40, 861, 862, 865, 867, 868, 873, 880, 889; A. pisum, UBC no. 826; A. aegypti, UBC nos. 812-7, 819-25; Philodina, UBC no. 819. 1 P. Abbot, unpublished data. 2 J. Russell, unpublished data. Buchnera is a vertically-transmitted, bacterial endosymbiont of aphids, which has proven useful in population-level studies in aphids. TrpEG typically shows faster evolution than mitochondrial COI/II. 3 S. Kaplan, unpublished data. 4 B. Birky, unpublished data.