Figure 6. The effect of diet on the hemolymph ecdysteroid titers of various stages of the CPB. Hemolymph was collected and extracts were prepared and analyzed as described in Materials and Methods. Late 4th, PP, +24 h, +48 h and +72 h = 4th instars whose color had begun to lighten, prepupae (absence of black pigmentation), burying +24 h, burying in the soil +48 h and burying +72 h, respectively. Each value represents the mean S. E. of at least four separate determinations.