Figure 5. Fractionation of Musca domestica female hemolymph proteins by KBr gradient ultracentrifugation. Hemolymph was collected from 6-day-old adult females and the plasma subjected to fractionation. Fractions (400 µl) were collected from the top to the bottom of the gradient. The density of each fraction was determined gravimetrically. Z9-C23:1 content of each hemolymph KBr fraction was determined by GC and is represented as a percentage of the total Z9-C23:1 in all fractions.