Figure 2. The effect of starvation after the critical period on the JHE activity profile of 5th instar Manduca sexta larvae. JHE activity was measured as nmoles of JH-acid produced per minute per ml of hemolymph. Each point is the mean of 7 to 9 pre-wandering larvae, or 4 to 6 post-wandering larvae. Vertical bars are standard errors of the mean (which in some cases do not exceed the size of the symbol). Larvae were starved at weights of 7 0.5 grams (dark vertical bar), and JHE activity was measured at 24-hour intervals thereafter. The expected JHE activity of normally feeding larvae (from Fig. 1) is indicated by the dotted line.